Iodine clock reaction

Read our standard health & safety guidance

Lesson organisation

This demonstration can be used at secondary level as an introduction to some of the ideas about kinetics. It can be used to stimulate discussion about what factors affect the rate of reaction. It also makes a useful starting-point for a student investigation.

As described this is intended as a demonstration, best done on a large scale for the most visual impact. The demonstration itself takes less than 1 minute. For a student investigation, the quantities required would be smaller but volumes then need to be measured quite accurately with, for example, disposable plastic syringes.

Apparatus and Chemicals

Eye protection
Balance (1 or 2 d.p.)
Beaker (1 dm³)
Beaker (250 cm³)
Volumetric flasks (1 dm³), 2
Measuring cylinder (50 cm³)
Measuring cylinders (100 cm³), 2
Stirring rod or magnetic stirrer and follower (optional)
Stopclock/timer

0.2g soluble starch
Glacial (concentrated) ethanoic acid (Corrosive), 30 cm³ (see note 3)
Anhydrous sodium ethanoate (sodium acetate, CH₃CO₂Na), 4.1 g (Low hazard)
Potassium iodide (KI), 50 g. (Low hazard)
Sodium thiosulfate-5-water (Na₂S₂O₃.5H₂O), 9.4 g (Low hazard) (see note 2)
20 volume hydrogen peroxide solution (H₂O₂(aq)), 500 cm³ (Irritant) (see note 3)
Deionised/distilled water, 2 dm³.

Technical notes

Glacial (concentrated) ethanoic acid is Corrosive. Refer to CLEAPSS Hazcard 38 (2007:38A)
20 vol hydrogen peroxide is Irritant. Refer to CLEAPSS Hazcard 30.

1 Solutions A and B should be made up before the demonstration. The solutions will keep overnight, but best results are obtained if the solutions are made up on the day.

2 Sodium thiosulfate will react with acids to give sulfur dioxide and a precipitate of sulfur, hence the sodium thiosulfate and ethanoic acid are separated in solutions A and B respectively.

3 Handling glacial ethanoic acid requires care (Corrosive) and should be done in a fume cupboard using gloves and eye protection.

If you have access to 1 mol dm^-3 dilute ethanoic acid, use 500 cm³ of this to make solution B. Mix 500 cm³ of 20 volume hydrogen peroxide with 500 cm³ of 1 mol dm^-3 ethanoic acid.

Procedure: Making up solutions A and B

HEALTH & SAFETY: Wear eye protection

Solution A is made up as follows:

a Make a paste of 0.2 g of soluble starch with a few drops of water in a beaker. Pour onto this approximately 100 cm³ of boiling water and stir.

b Pour the resulting solution into a 1 dm³ beaker and dilute to around 800 cm³.

c Add 4.1 g of sodium ethanoate, 50 g of potassium iodide and 9.4 g of sodium thiosulfate. Stir until all the solids have dissolved and allow to cool to room temperature.

d Pour the mixture into a 1 dm³ volumetric flask and make up to 1 dm³ with water.

Solution B is made up as follows:

e In a 1 dm³ volumetric flask mix 500 cm³ of 20 volume hydrogen peroxide with 30 cm³ of glacial ethanoic acid and dilute to 1 dm³ with water. (See Technician notes about using dilute ethanoic acid as a less hazardous alternative)

Both solutions are colourless although solution A will be slightly cloudy.

The demonstration

f Measure 100 cm³ of solution A and 100 cm³ of solution B in separate 100 cm³ measuring cylinders.

g Pour both solutions simultaneously into a 250 cm³ beaker to mix. Ensure thorough mixing with a stirring rod or magnetic stirrer.

h After about 20 seconds at room temperature the mixture will suddenly turn dark blue.

The appearance of the blue colour may be timed – an assistant/student can start and stop the timer.

Additional notes

1 Hydrogen peroxide is capable of oxidising thiosulfate ions to tetrathionate
ions but the reaction is too slow to affect this demonstration.

2 The ethanoic acid/sodium ethanoate is added to buffer the pH.

3 The acid will react slowly with sodium thiosulfate and produce a cloudy suspension of sulfur and release sulfur dioxide which is TOXIC – refer to CLEAPSS Hazcard 97. To avoid this the acid and sodium thiosulfate are separated in solutions A and B.

4 The CLEAPSS Guide L195 ‘Safer chemicals, safer reactions’ gives useful information about how to carry out rates experiments involving acid/thiosulfate mixtures more safely.

Teaching notes

Visual tips: a white background will help so that the impact of the sudden and spectacular colour change is not lost. Scaling up the volumes of solution that are mixed may help in a large room.

There is no warning of when the blue colour is about to appear.

It may help understanding if the students are already familiar with the reactions of starch and iodine, and iodine and sodium thiosulfate, so it may be worth demonstrating these beforehand.

The basic reaction is:

H₂O₂(aq) + 2I^– (aq) + 2H⁺ (aq) → I₂(aq) + 2H₂O(l)

[For more advanced discussions or investigations - this reaction is the rate determining step and is first order with respect to both H₂O₂ and I^–.]

As soon as the iodine is formed, it reacts with the thiosulfate to form tetrathionate ions and recycles the iodide ions by the fast reaction:

2S₂O₃^2–(aq) + I₂(aq) → S₄O₆^2–(aq) + 2 I^– (aq)

As soon as all the thiosulfate is used up, free iodine (or, strictly, I₃^- ions) remains in solution and reacts with the starch to form the familiar blue-black complex.

The time for the blue colour to appear can be adjusted by varying the amount of thiosulfate in solution A so a ‘clock’ of any desired time interval can be produced.

If the demonstration is being done for entertainment, the imaginative teacher will be able to think up some suitable patter.

Health and Safety checked, November 2006